Fig. 2

Enrichment of pro-inflammatory and pro-fibrotic transcripts in IPF versus normal T cells. Normal T cells were magnetically sorted from peripheral blood of normal donors and IPF T cells were sorted form IPF lung explant cellular suspensions, RNA was extracted and subject to RNA sequencing. Normalized FPKM values and EDGE significant transcripts were calculated using CLC workbench genomics and imported into Morpheus (Broadinstitute.org). a Depicted are significantly differentially expressed transcripts in IPF relative to normal T cells clustered using one minus Pearson correlation distance metric. b–d Depicted are heat maps generated using Morpheus of FPKM values for transcripts encoding proteins involved in various pro-inflammatory and pro-fibrotic pathways (b), T-cell activation and polarization (c), and in lysosomal genesis and function (d). n = 3 per group