Fig. 4
From: A built-in adjuvant-engineered mucosal vaccine against dysbiotic periodontal diseases
Detection of cognate antigens. Immunofluorescence detection of the natural form of tFomA or Hgp44 on the surface of live F. nucleatum (a) or P. gingivalis (b), respectively. Fresh cultures of F. nucleatum ATCC 10953 or P. gingivalis ATCC 33277 were applied on the glass slides and then incubated with pre-immune, anti-Fn or anti-divalent vaccine pooled sera. The specimens were examined under confocal microscopy. Detection of specific protein band(s) by the anti-divalent sera in the clinical isolates of F. nucleatum or P. gingivalis. Bacterial lysates of F. nucleatum (c) or P. gingivalis (d) were analyzed by western blotting with anti-divalent sera. Fn F. nucleatum, Fs F. simiae, Fh F. hwasookii, Fp F. periodonticum, Pg P. gingivalis, Pe P. endodontalis. ATCC Americal Type Culture collection, KCOM Korean Collection for Oral Microbiology
