Fig. 2

Increased numbers of secretory intestinal epithelial cells in VillinCre_STAT6vt mice. a Paraffin sections from the duodenum, jejunum, and ileum of naive VillinCre_STAT6vt mice and their control littermates were stained for secretory intestinal epithelial cells. From left to right: PAS and H&E staining for goblet cells (purple), anti doublecortin-like kinase 1 (DCLK1) staining for tuft cells (orange), and anti-Lysozyme staining for Paneth cells (red). For tuft and Paneth cells, the nuclei were counter stained with DAPI (blue). Magnified views of the cells of interest are in the upper right corner of each picture. Scale bar represents 50 µm. b mRNA expression of genes associated with secretory intestinal epithelial cells. From left to right: Retnlb (Relm-β, goblet cells), interleukin-25 (IL-25, tuft cells), angiogenin-4 (Ang4, Paneth cells). PBGD and HPRT1 served as reference genes for normalization. c Western blot for Relm-β protein out of fecal pellets of one experiment, #1 and #2 represent individual mice per group. Pictures are representative of one out of at least two independent experiments, whereas the bar graphs show the mean + s.e.m. of pooled data from two independent experiments. Two to three mice per group per experiment were used. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, unpaired two-tailed Student’s t test