Fig. 3

Tuft cell amplification in mice with constant STAT6 signaling in IECs does not lead to increased influx of ILC2s. a Cryosections from duodenum of wild-type mice on day 7 after primary infection with Nippostrongylus brasiliensis (left) and ileum of naive VillinCre_STAT6vt mice (middle) and their control littermates (right) stained for killer cell lectin-like receptor subfamily G, member 1 (KLRG1, red), DCLK1 (green), and CD3 (turquoise). Scale bars represent 20 µm. b Representative FACS plots of naive VillinCre_STAT6vt mice and their control littermates analyzed for KLRG1⁺CD90⁺ ILC2s gated from Lin⁻CD4⁻ cells in the intestinal lamina propria. Lin⁺ cells include CD3e⁺ CD8e⁺ CD11b⁺ CD49b⁺ B220⁺ Gr1⁺ Ter119⁺ TCRγδ⁺ NK1.1⁺ cells. The bar graphs show the percentage and the total cell count of KLRG1⁺CD90⁺ ILC2s of living CD45⁺ cells. c Representative FACS plots of naive VillinCre_STAT6vt and their control littermates analyzed for SSC-A^hi Siglec-F⁺ eosinophils gated from living CD45⁺ cells or Ly6-C⁺ Ly6-G⁺ neutrophils and CD49b⁺ CD200R3⁺ basophils gated from living CD45⁺ Siglec-F⁻ cells or CD45⁺ CD4⁺ T cells gated from living cells. The bar graphs show the percentage of eosinophils, neutrophils, basophils, and CD4⁺ T cells of living CD45⁺ cells. a, b Representative pictures and plots of one out of two independent experiments and plots of one experiment (c) are depicted. Bar graphs show the mean + s.e.m. of pooled data from two independent experiments a, b or the mean + s.d. from one experiment c. Two to three mice per group per experiment were used