Fig. 5

Faster parasite clearance and normal Th2 response after Nb infection. VillinCre_STAT6vt mice and their control littermates were infected with Nb L3. a Number of eggs in fecal pellets on days 6, 8, and 10 after infection and number of adult worms in the intestinal lumen on day 8 after infection. b Number of adult worms in the intestinal lumen on day 10 after infection of mice that had been depleted of CD4 T cells (α-CD4) or not (Iso-Ctrl). c Immune cells were isolated out of the LP on day 8 after Nb infection and analyzed by FACS. The bar graphs show the percentages of Siglec-F⁺ eosinophils from living CD45⁺ cells and CD49b⁺ CD200R3⁺ basophils and Ly6-C⁺ Ly6-G⁺ neutrophils from living CD45⁺ Siglec-F⁻ cells. CD90⁺ KLRG1⁺ Lin⁻ ILC2s were gated from living CD45⁺ Lin⁻ cells. d mRNA expression of genes associated with a type 2 immune response on day 8 after Nb infection. HPRT served as the reference gene for normalization. e Intracellular staining for IL-4 and IL-13 production by CD4⁺ T cells from mesenteric lymph nodes of day 10 Nb-infected mice. Dot plots are gated on single live CD4⁺ cells and gates of the stimulated samples were set according to the respective unstimulated control. Bar graphs show the frequency of IL-4-positive IL-13-negative, IL-4/IL-13 double positive, and IL-4-negative IL-13-positive CD4⁺ T cells. f Amounts of IL-4 and IL-13 in the supernatant of anti-CD3/anti-CD28 restimulated T cells quantified by ELISA. g IgE levels in the serum of day 10 Nb-infected mice quantified by ELISA. a–g Black symbols/bars represent VillinCre_STAT6vt mice, gray symbols/bars represent their control littermates, and white columns represent WT C57BL/6 mice. a, b, e–g Bars and symbols show the mean + s.e.m of pooled data from at least two independent experiments. a (worm count day 8), c, d Bars show the mean + s.d. from one experiment. e Dot plots are representative of one out of two independent experiments. Two to four mice per group per experiment were used for the experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, unpaired two-tailed Student’s t test