Fig. 2
From: Regulation of HMGB1 release protects chemoradiotherapy-associated mucositis
NecroX-7 reduced oxidative stress, mitochondrial dysfunction, and apoptosis in 5-fluorouracil (5-FU)-induced oral mucositis (OM). Reactive oxygen species (ROS) accumulation in chemotherapy-induced OM. a Representative fluorescence photomicrographs of sections of mouse tongue mucosa labeled with dihydroethidium (DHE; red) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue) to show nuclei. DHE fluorescence intensity is shown. The NecroX-7-treated group showed significantly reduced ROS levels. Original magnification, ×50 and ×200. b Representative confocal images of γH2AX (red) staining in the ventral tongue. Blue, DAPI. Arrows indicate rH2A.X+ cells, and numbers of foci in epithelial cells are plotted. c, d Quantification of the staining in a and b. e Transmission electron microscopy analysis of mitochondrial morphology in the tongue of vehicle- or NecroX-7-treated mice with OM. The NecroX-7-treated group exhibited reduced mitochondrial dysfunction and apoptosis. Arrows indicate abnormal mitochondria. Scale bars, 0.5 and 100 μm. N7 NecroX-7. **P < 0.01, ***P < 0.001
