Fig. 3
From: Regulation of HMGB1 release protects chemoradiotherapy-associated mucositis
High-mobility group box 1 (HMGB1) inhibition by NecroX-7 protected chemotherapy-induced mucositis. Mice were euthanized 1, 4, 7, and 10 days after the first injection of 5-fluorouracil (5-FU) and tongue tissues were collected for analysis. a HMGB1 mRNA levels were measured at the indicated times by real-time polymerase chain reaction using β-actin for normalization. b HMGB1 translocation. HMGB1 localization was visualized by immunohistochemistry. Brown, HMGB1; blue, nuclei stained with hematoxylin; arrows indicate cytoplasmic HMGB1 staining. The two black boxes in the upper panels (×400) are shown at higher magnification in the lower panels (×1000). c The percentage of immune cells with only cytoplasmic HMGB1 staining among total number of tissues was calculated. Representative images from six mice per group are shown. d Poly-ADP-ribose polymerase (PARP) and HMGB1 levels were analyzed by western blotting, using α-tubulin as the loading control. Two independent experiments were performed. N7 NecroX-7. **P < 0.01
