Fig. 4
From: Regulation of HMGB1 release protects chemoradiotherapy-associated mucositis
NecroX-7 protect oral mucositis from increased high-mobility group box 1 (HMGB1) release and p53-upregulated modulator of apoptosis (PUMA). Mice were euthanized 1, 4, 7, and 10 days after the first injection of 5-fluorouracil (5-FU) and tongue tissues were collected for analysis. a Phospho-p53 (Ser15) and p53 protein levels analyzed by western blotting, using α-tubulin as the loading control. Two independent experiments were performed. b PUMA mRNA levels were measured at the indicated times by real-time polymerase chain reaction using β-actin for normalization. c p53 localization was visualized by immunohistochemistry. Brown, p53; blue, nuclei stained with hematoxylin. Original magnification, ×300 and ×600. d Caspase-3, Bax, and Bcl-2 expression levels were determined in tongue tissues by real-time PCR using β-actin for normalization. N7 NecroX-7. *P < 0.05, **P < 0.01
