Fig. 6
From: Regulation of HMGB1 release protects chemoradiotherapy-associated mucositis
Effect of NecroX-7 on the oral and intestinal mucosa of mice receiving 5-fluorouracil (5-FU) and total body irradiation (TBI). a Experiment protocol. Oral and gastrointestinal mucositis were induced in mice using 5-FU (50 mg/kg, qdx2), followed by TBI (8 Gy, single fraction). NecroX-7 (30 mg/kg, intravenous) or phosphate-buffered saline was administered once daily for 5 days. Morphological changes were evaluated by hematoxylin and eosin (H & E) staining on day 10 after initiation of 5-FU and TBI. b Representative H & E-stained micrographs of the dorsal (upper panel) and ventral (lower panel) tongue. Original magnification, ×400. c Tongue mucosal epithelium thickness. d H & E-stained images of mouse small intestine (upper panel) and lung (lower panel). Original magnification, ×50. e Crypt depth and f intestinal villus height. NAC N-acetylcysteine, N7 NecroX-7. Results are representative of two independent experiments (n = 5–6 mice/group). *P < 0.05, **P < 0.01, ***P < 0.001
