Fig. 3

PAF is released in response to intestinal mucosal injury to promote repair. a PAF levels in 3-mm punch biopsies of resealing colonic wounds on different post-injury days compared to intact tissue analyzed by lipodomics (n = 3). b–d Wound areas of scratch wounded SKCO15 IEC monolayers, incubated with various treatments were continuously imaged. Percent wound closure was calculated by comparison of 0 and 18 or 24 h postinjury. Wounded IECs treated with b PAF (10 μM) with or without PAFR antagonist PCA 4248 (10 μM) for 24 h; c IFNγ (100 ng/ml), TNFα (100 ng/ml), and PAF alone or in combination for 24 h; d IFNγ, TNFα, and PCA 4248 alone or in combination for 18 h. e Wounded primary IECs treated with PAF, IFNγ (100 ng/ml), and TNFα (100 ng/ml), and with or without PAFR antagonist PCA 4248 (10 μM) for 12 h. Experiments were repeated three times, results of one representative experiment are shown. Statistical comparisons performed using one- or two-way ANOVA with Bonferroni’s multiple comparison (*p < 0.5; **p < 0.01; ***p < 0.001; mean ± SEM). IT intact tissue, NT vehicle control