Fig. 6

Inhibition of TNFα signaling in vivo ablates PAFR upregulation after injury and delays intestinal mucosal wound healing. a Tnfa and b Ifng mRNA levels in 3-mm punch biopsies of resealing colonic wounds compared to intact tissue analyzed by qPCR (n = 3). c Ptafr mRNA levels in 3-mm punch biopsies of resealing colonic wounds isolated from mice treated with a TNFα neutralizing antibody or control on different days postinjury compared to intact tissue analyzed by qPCR (n = 3). d Endoscopic images of colonic mucosal wounds 1 and 3 days after biopsy injury in C57BL/6J mice treated with a TNFα neutralizing antibody or IgG control. Graph shows quantification of wound closure (n = 5). Statistical comparisons were performed using one-way ANOVA with Bonferroni’s multiple comparison and unpaired two-tailed Student’s t test with Welch’s correction (*p < 0.5; ***p < 0.001; mean ± SEM). IT intact tissue, Ctl control