Fig. 2

TRAIL inhibited a panel of proinflammatory cytokines and chemokines in inflamed colon tissues of mice with DSS-induced colitis. a In the group with DSS-induced colitis, C57BL/6 mice were fed 2.5% DSS in sterilized drinking water for 5 days (days 0–5), followed by 2 days of normal water (days 6 and 7) and treated with either vehicle (200 μl/mouse/day, i.p.) or TRAIL (50 μg/mouse/day, i.p.) from day 0. Control mice were given normal drinking water only (n = 15 for each group). On day 7, colon tissues were moved from each group, and tissue lysates were analyzed by a cytokine array. Representative cytokine array data are shown (left panel) and quantified (right panel). Data are presented as the mean ± SD of triplicate samples. *p < 0.05 by Student’s t test, compared to DSS-induced colitis mice treated with the vehicle. b On day 7, colon tissue lysates were prepared from each group, and specific cytokine levels, including interleukin (IL)-33, TNF-α, interferon (IFN)-γ, IL-17, and IL-10 were assayed by ELISAs. Data are presented as the mean ± SD of triplicate samples. *p < 0.05, **p < 0.01 by Student’s t test. Data are representative of at least three independent experiments. c In the group with DSS-induced colitis, C57BL/6 mice were fed 2.5% DSS in sterilized drinking water for 5 days (days 0–5), followed by 2 days of normal water (days 6 and 7) and treated with either vehicle (200 μl/mouse/day, i.p.) or TRAIL (50 μg/mouse/day, i.p.) from day 4. Control mice were given normal drinking water only. Body weight changes (% original body weight) in each group were recorded, and results are presented as the mean ± SD (n = 15 in each group). *p < 0.05, **p < 0.01 by Student’s t test, compared to DSS-induced colitis mice treated with TRAIL