Fig. 3 | Mucosal Immunology

Fig. 3

From: Flavonoid VI-16 protects against DSS-induced colitis by inhibiting Txnip-dependent NLRP3 inflammasome activation in macrophages via reducing oxidative stress

Fig. 3

VI-16 inhibited the activation of caspase-1 in colonic macrophages rather than in intestinal epithelial cells. a Production of IL-1β and IL-18 in serum of DSS-induced colitis mice were determined by ELISA. b Immunoblot analysis of IL-1β expression in colonic macrophages. c Production of IL-1β and IL-18 in intestinal epithelial cells were determined by ELISA. d Immunoblot analysis of IL-1β expression in intestinal epithelial cells. e Bone marrow chimeras were generated with wild-type (WT) and NLRP3−/− mice. Immunoblot analysis of IL-1β expression in colonic macrophages and intestinal epithelial cells of DSS-treated bone marrow chimeras mice. Data are presented as mean ± SD. **P < 0.01 compared with normal group; #P < 0.05, ##P < 0.01 compared with DSS group

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