Fig. 7

VI-16 inhibited the dissociation of Txnip from Trx-1 and to binding to NLRP3 by improving the antioxidant capacity. The protein expression (a) and mRNA levels (b) of Txnip and Trx-1 were detected in LPS-primed differentiated THP-1 cells treated with indicated concentration of VI-16, followed by incubation with ATP (5 mM) for 1 h. c Confocal microscopy of LPS-primed differentiated THP-1 cells, immunostained for NLRP3 (green) and Txnip (red). Scale bars, 10 µm. d Immunoblot analysis of Txnip immunoprecipitates in LPS-primed differentiated THP-1 cells, followed by incubation with ATP (5 mM) for 1 h, nigericin (4 μM) for 3 h or MSU (150 µg/ml) for 6 h, then probed for NLRP3, Trx-1, and Txnip. e Levels of the ROS were measured in LPS-primed differentiated THP-1 cells. Data are presented as mean ± SD. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with ATP group