Fig. 4 | Mucosal Immunology

Fig. 4

From: Colitis susceptibility in mice with reactive oxygen species deficiency is mediated by mucus barrier and immune defense defects

Fig. 4

Nox inactivation impairs the colonic mucus layer. Relative expression of colonic Muc2, Fut1, and Fut2 mRNA (a). Representative immunofluorescence images of fixed mouse colon probed with Muc2 (green), UEA-1 (green), and DAPI (blue) (b, c). Representative high-resolution images of mouse colon (d) with dense mucus layer (red bar) and presence of bacteria inside crypts (arrows); mucus layer thickness (e) and goblet cell granule size (f) quantification. Data are presented as mean ± SEM. Each symbol represents an individual mouse; n = 7–10 mice (a); n = 4–5 mice (b, c); n = 9–10 colon sections from three 15-week-old mice for d–f. Data were analyzed using unpaired t-test. **P < 0.01 and ***P < 0.001 vs WT. Scale bar 100 µm (b, c) and 20 µm (d)

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