Fig. 4 | Mucosal Immunology

Fig. 4

From: Regulating colonic dendritic cells by commensal glycosylated large surface layer protein A to sustain gut homeostasis against pathogenic inflammation

Fig. 4

Transcriptomic programming of colonic DCs by P. UF1 expressing glycosylated LpsA during Listeria infection. C57BL/6 mice were gavaged with P. UF1 or ΔlspA P. UF1 and then orally infected with ΔactA L. m. Colonic CD11chi MHCIIhi CD11b+ F4/80− DCs were FACS sorted for transcriptomic analysis seven days after infection. a Heatmap showing significantly differentially expressed genes (FDR P < 0.05, fold change ≥ 1.5) in DCs derived from mice gavaged with P. UF1 compared to ΔlspA P. UF1. b qRT-PCR analysis of a selection of differentially expressed genes. Results are presented as fold change over DCs of P. UF1-gavaged mice. c GSEA showing differentially enriched pathways. Number signs (#) indicate gene sets without FDR correction (P < 0.05). Data are from 1 experiment (n = 4 mice/group). Error bars indicate SEM. *P < 0.05, **P < 0.01, two-tailed unpaired t test

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