Fig. 5
Metabolomic programming of colonic DCs by P. UF1 expressing glycosylated LspA during Listeria infection. a Colonic DCs were enriched from mice gavaged with P. UF1 or ΔlspA P. UF1 and orally infected with ΔactA L. m. The mitochondrial respiration of enriched DCs by magnetic beads was evaluated by measuring their real-time changes in the ECAR and OCR in response to sequential addition of oligomycin (Oligo), FCCP, rotenone (Rot), and antimycin A (Ant). Bar graphs show the basal ECAR and OCR. b Colonic CD11chi MHCIIhi CD11b+ F4/80− DCs were FACS sorted from mice gavaged with P. UF1 or ΔlspA P. UF1 and orally infected with ΔactA L. m. The cellular metabolome of DCs was analyzed by high-resolution mass spectrometry. Heatmap showing the distinctive metabolomes of colonic DCs. c Metabolic pathway analysis of metabolites with intensities significantly altered by comparing FACS sorted DCs derived from P. UF1- and ΔlspA P. UF1-gavaged mice. Dashed line shows the permutation P value of 0.05. d Scatter plots for selected metabolite features, with putative annotation, in the significant pathways identified by Mummichog. The m/z, retention time (in seconds) and adduct ion were labeled for each metabolite. Data are from 1 experiment (n = 4–5 samples/group). Error bars indicate SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired t test
