Fig. 6

Requirement of glycosylated LspA for protective T cell regulation. Signr1^+/+ and Signr1^−/− mice were gavaged with P. UF1, ΔlspA P. UF1 or PBS and then orally infected with ΔactA L.m. Seven days post infection, colonic DC and T cell responses were analyzed. a Percentages and total counts of IL-1β⁺, IL-6⁺, and IL-12/23p40⁺ DCs in Signr1^+/+ (top panel) and Signr1^−/− mice (bottom panel). Flow plots are from Signr1^+/+ mice. b Percentages and total counts of Th17, IL-10⁺ Th17, and IL-10⁺ FoxP3⁺ Treg cells in Signr1^+/+ (top panel) and Signr1^−/− mice (bottom panel). Flow plots are from Signr1^+/+ mice. c Box and whisker plots of fecal ΔactA L. m burdens measured in Signr1^+/+ (left panel) and Signr1^−/− mice (right panel) on days 1–4 after infection. Dashed lines represent the limit of pathogen detection. d Box and whisker plot showing transcript levels of proinflammatory molecules in the colonic tissues of Signr1^+/+ mice. Data are representative of 2 (Signr1^−/− mice) or 3 (Signr1^+/+ mice) independent experiments (n = 5 mice/group, a, b) or pooled from 2 independent experiments (n = 9–10 mice/group, c, d). Error bars indicate SEM. *P < 0.05, **P < 0.01, ANOVA plus Tukey’s post-test (a, b) or Kruskal-Wallis plus Dunn’s post-test (c, d)