Fig. 2

Assessment of IEC-specific immune and barrier functions and systemic inflammation in 12-month-old Rabgef1^IEC-KO mice. a IECs were isolated from Rabgef1^IEC-KO and littermate control mice and expression of the indicated genes was assessed by RT-qPCR (n = 4 mice/group). b Quantification of intestinal permeability: serum levels of FITC fluorescence 4 h after FITC-dextran gastric gavage (n = 6–12 mice/group). c Cytokine concentrations measured by Luminex assay in the sera of mice (n = 7–8 mice/group). Concentration values below the detection threshold are depicted as zero values. Data are shown as mean + SEM and are pooled from 2 independent experiments, each of them giving similar results. P values were calculated using a two-way (a, c) or a one-way (b) ANOVA with Tukey’s post hoc tests. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns not significant (P > 0.05). FITC fluorescein isothiocyanate