Fig. 4
Contribution of epithelial RABGEF1 to the regulation of IEC innate responses, intestinal permeability, and systemic inflammation in IL-10-deficient mice. Comparisons between 3-to-4-month-old Rabgef1IEC-KO and Rabgef1fl/fl (control) mice raised on the Il10−/− background are shown. a IECs were isolated and expression of the indicated genes was assessed by RT-qPCR (n = 4 mice/group). b Quantification of intestinal permeability: serum levels of FITC fluorescence 4 h after FITC-dextran gastric gavage (n = 6 mice/group). c Cytokine concentrations measured by Luminex assay in the sera of mice (n = 6–7 mice/group). Concentration values below the detection threshold are depicted as zero values. Data are shown as mean + SEM, as well as individual values in (g), and are pooled from two independent experiments, each of them giving similar results. P values were calculated by a two-way (a, c) or a one-way (b) ANOVA with Tukey’s post hoc tests. *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant (P > 0.05). FITC fluorescein isothiocyanate
