Fig. 7 | Mucosal Immunology

Fig. 7

From: Epithelial RABGEF1 deficiency promotes intestinal inflammation by dysregulating intrinsic MYD88-dependent innate signaling

Fig. 7

Endosomal abnormalities in RABGEF1-deficient IECs in vitro. Immortalized primary mouse IECs transduced with lentiviral plasmids encoding nontarget (NT) and Rabgef1 single guide (sg)RNA and Cas9 (sgRNA NT and sgRNA Rabgef1, respectively) were used. a High-resolution confocal microscopy pictures of Rab5 (red) and DAPI (blue) staining in IECs, at steady-state and 1 h after LPS stimulation (100 ng/ml). Pictures are representative of similar results obtained in each of two experiments performed with two different batches of cells. b Representative Western Blots and c protein quantification showing the levels of EEA-1 0 and 60 min after LPS stimulation. Data are shown as mean ± SEM, and are pooled from independent experiments performed with three separate batches of cells. P values were calculated using a one-way ANOVA test followed by Tukey’s post hoc tests for multiple comparisons. ***P < 0.001; ns not significant (P > 0.05)

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