Fig. 8

RABGEF-dependent regulation of p38-dependent signaling and chemokine production in IECs. Immortalized primary mouse IECs transduced with lentiviral plasmids encoding nontarget (NT) and Rabgef1 single guide (sg)RNA and Cas9 (sgRNA NT and sgRNA Rabgef1, respectively), and RABGEF1-deficient IECs (sgRNA Rabgef1) transduced with plasmids encoding Rabgef1 to restore RABGEF1 expression (Rescued RABGEF1) were used. a Representative Western Blots and b protein quantification showing the levels of the indicated phospho-proteins and total proteins at different times following LPS stimulation. Results are representative of similar results obtained in each of three experiments performed with three separate batches of cells. c Cytokine concentrations measured by Luminex assay in the supernatants of media (Vehicle)- or LPS-stimulated IECs. Data are mean + SEM and are pooled from two independent stimulation experiments performed with two separate batches of cells. P values were calculated using a two-way ANOVA test followed by Tukey’s post hoc tests for multiple comparisons. ^**P < 0.01; ^***P < 0.001; nd not detected