Fig. 6 | Mucosal Immunology

Fig. 6

From: Limited recognition of Mycobacterium tuberculosis-infected macrophages by polyclonal CD4 and CD8 T cells from the lungs of infected mice

Fig. 6

BCG elicits T cells that recognize Mtb-infected macrophages. a Splenic T cells were enriched by negative selection from the age-matched control (open symbols) or BCG-vaccinated (closed symbols) mice, 4−5 weeks after immunization. The MIME assay was used to determine the frequency of T cells that recognized Mtb-infected macrophages. In addition, the frequency of Mtb epitope-specific T cells among these T cells was determined by coculture with uninfected macrophages and the respective peptides. Data are combined showing three individual mice per experiment from 2 to 3 experiments (different symbols) (n = 9 mice for MIME response; n = 6 mice for peptide response). Each point is an average of duplicate two replicates. b BCG-vaccinated or control C57BL/6 mice (n = 7/group) were challenged 9 months post vaccination using aerosol Mtb infection and CFU in the lungs and spleens was assessed at 4 wpi. Data from one representative experiment are shown

Back to article page