Fig. 4

HlyA enhances bacterial invasion into renal epithelial cells. a, b Intracellular bacteria in 786-O cells infected with CFT073, ΔhlyA or ∆hlyA p-hlyA alone (a), or infected with ∆hlyA (MOI 0.01) in combination with dialysis buffer or HlyA (b) at 6 hpi were analyzed by killing extracellular bacteria using gentamicin (n = 3, three independent experiments each with two replicate wells). c Secreted GM-CSF of 786-O cells treated with dialysis buffer, HlyA or pro-HlyA (75 nM) for 12 h after incubation with DMSO or 20 μM GI254023X for 20 h (n = 3, three independent experiments). d Intracellular bacteria in 786-O cells infected with CFT073 or ΔhlyA (MOI 0.01) at 6 hpi after incubation with DMSO or GI254023X (20 μM) for 20 h (n = 3, three independent experiments). e, f Intracellular bacteria in 786-O (e) or HK-2 (f) cells infected with ΔhlyA (MOI 0.01) in combination with dialysis buffer or HlyA at 6 hpi after incubation with DMSO or GI254023X (20 μM) for 20 h (n = 3, three independent experiments). Data are the mean ± SD, one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001