Fig. 6

HlyA interacts with Nectin-2. a Co-immunoprecipitations analysis of the interaction between FLAG-tagged HlyA and Myc-tagged Nectin-2 expressed in 293T cells. b Co-immunoprecipitations analysis of the interaction between FLAG-tagged HlyA and Nectin-2 in 786-O cells. c Co-immunoprecipitations analysis of the interaction between purified FLAG-tagged HlyA and Nectin-2 protein. α, anti-. d HA-tagged HlyA and Myc-tagged Nectin-2 interactions in 293T cells analyzed by immunofluorescence assays. Scale bar, 10 μm. Blue, nucleus; red, HA-tagged HlyA; Green, Myc-tagged Nectin-2. e A proposed model that HlyA induces GM-CSF-mediated acute kidney injury