Fig. 2 | Mucosal Immunology

Fig. 2

From: Entamoeba histolytica stimulates the alarmin molecule HMGB1 from macrophages to amplify innate host defenses

Fig. 2

Eh in contact with macrophage induces cytoplasmic translocation of HMGB1. BMDMs were stimulated with Eh for 10 min and translocation of nuclear HMGB1 was measured by immunofluorescence analysis. a Representative images of HMGB1 immunostaining in control and Eh-exposed macrophages from three independent experiments (scale bar: 10 μm). The white arrows in the DIC images show the point of contact of Eh with macrophages (Mɸ). b The mean intensity of the cytoplasmic HMGB1 staining in control and Eh-infected macrophages are shown as mean ± SEM. Statistical significance was determined by t test. ***P < 0.001.

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