Fig. 2: Goblet cells support antigen presenting cell acquisition of luminal antigen and CD4+ T cell responses to luminal antigen in the gut draining lymph nodes.
From: Goblet cell associated antigen passages support the induction and maintenance of oral tolerance
a 4kD and 40kD FITC-dextran in serum after oral gavage in Math1f/fvil-Cre-ERT2 mice and Math1f/f littermate controls. b, c Luminal Ova acquisition by SI LP-APCs assessed by flow cytometry 2 h post oral gavage. d Antigen presentation capacity of SI LP-APCs isolated from mice Math1f/f and Math1f/fvil-Cre-ERT2 mice given luminal Ova as assessed by expansion of Ova-specific OTII T cells in ex vivo cultures. e Histograms and quantification of in vivo proliferation of CFSE-labeled OTII T cells in SI draining MLN of Math1f/f and Math1f/fvil-Cre-ERT2 mice 2 days after oral Ova gavage. f Antigen acquisition by distal colon LP-APCs assessed by flow cytometry 2 h following intra-colonic administration of fluorescent Ova. g Histograms and quantification of in vivo proliferation of CFSE-labeled OTII T cells in distal colon draining caudal LN of Math1f/f and Math1f/fvil-Cre-ERT2 mice 2 days after Ova enema. Data are representative of two or more replicates with ≥3 mice per group, each data point represents an individual mouse. Data is presented as mean ± SEM, *P < 0.05, ns not significant.
