Fig. 6: GAPs support tolerance to dietary antigen in the SI and tolerance to luminal antigens in the distal colon.

a Quantification and b images of footpad swelling following the induction of oral tolerance by dietary Ova, Ova immunization, and Ova footpad challenge in mice lacking goblet cells (Math1^f/f vil-Cre-ER^T2 mice) and their littermate controls. Quantification of footpad swelling following dietary Ova, Ova immunization, and Ova footpad challenge in c mice lacking EGFR in goblet cells (EGFR^f/fMath1^Cre*PR mice) and their littermate controls treated with mEGF and in d mice lacking mAChR4 in goblet cells (mAChR4^f/f Math1^Cre*PR mice) and littermate controls. e Quantification of footpad swelling following the induction of tolerance by Ova enema, Ova immunization, and Ova footpad challenge in mice lacking goblet cells (Math1^f/f vil-Cre-ER^T2 mice) and their littermate controls. f–i Serum IFNγ in mice treated as in a-e 24 h following footpad challenge. *P < 0.05. Data are presented as the mean ± SEM. Each data point represents an individual mouse.