Fig. 6: Interaction of IgA preparations with E. coli O55.
a Individual fractions and modifications of SIgA at 2 mg/ml were titrated and incubated with 2 × 109 CFU/ml of E. coli O55 at 37 °C followed by overnight incubation at 4 °C. The reciprocal of the highest dilution giving visible agglutination is presented as agglutination titer. Resulting values are from three independent experiments. b E. coli O55 were incubated with SIgA preparations conjugated with DyLight 488, washed, and analyzed by flow cytometry. After Fab deglycosylation, binding to E. coli O55 was reduced to 67% of its original value. Means ± SD are provided. P values were calculated using Kruskal-Wallis test with Dunn-Bonferroni post-hoc test. *p < 0.05.
