Fig. 1: Baseline colonic phenotype in Mdr2−/− mice with active liver disease. | Mucosal Immunology

Fig. 1: Baseline colonic phenotype in Mdr2−/− mice with active liver disease.

From: Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis

Fig. 1

WT and Mdr2−/− mice were euthanized at age 6, 10, and 30 weeks. Feces and colon tissues were collected. a Levels of fecal bile acids from 10-week-old WT and Mdr2−/− mice were measured using mass spectrometry. b, c Representative colon histology (hematoxylin and eosin [H&E]; 200×) and histological scores. Positive control in c is WT/DSS from Fig. 2e. d Colonic barrier function in 10-week-old mice was assessed by FITC-dextran gavage. Serum levels of FITC-dextran were measured 4 h post gavage. Positive control is WT/DSS from Fig. 2g. e Colon tissue was homogenized and cytokine protein levels were measured. n ≥ 4 mice per group. Data are expressed as means ± SEM, two-way analysis of variance.

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