Fig. 2: Induction of DSS colitis in Mdr2−/− mice with established cholestatic liver disease. | Mucosal Immunology

Fig. 2: Induction of DSS colitis in Mdr2−/− mice with established cholestatic liver disease.

From: Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis

Fig. 2

Ten-week-old WT and Mdr2−/− mice were subjected to 1.5% dextran sodium sulfate (DSS) colitis or water control (diH2O) for 5 days and then allowed to recover. Tissues and sera were collected upon euthanasia on day 9. a Scheme of treatment. b Body weight curves over the course of DSS treatment and recovery. c Disease activity index (DAI) measured by combining weight loss, stool consistency, and bleeding. d, e Representative colon histology (H&E; 100×) and histological scores. f Colon length at time of euthanasia divided by baseline body weight. g Colonic barrier function in 10-week-old mice was assessed by FITC-dextran gavage. h Colon tissue was homogenized, and cytokine protein levels were measured. n ≥ 10 mice per group. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-way analysis of variance.

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