Fig. 3: Colonic expression of adhesion molecules in DSS-treated WT and Mdr2−/− mice. | Mucosal Immunology

Fig. 3: Colonic expression of adhesion molecules in DSS-treated WT and Mdr2−/− mice.

From: Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis

Fig. 3

WT and Mdr2−/− mice were subjected to DSS treatment as described in Fig. 2. a Real-time quantitative PCR (qPCR) analysis of colon tissue. b ELISA measurement of colonic MAdCAM-1 protein. c, d Representative immunofluorescence double staining of MAdCAM-1 (red; Alexa Fluor 555) and CD31 (green; Alexa Fluor 488) on colon tissue (100×) shown in d. Quantitation of MAdCAM-1 density normalized to CD3 is shown in c. n ≥ 5 mice per group. Data are expressed as means ± SEM. *P < 0.05, ***P < 0.001, two-way ANOVA.

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