Fig. 4: Hypoxia induces HIF-1α-RORγt interaction and transcriptional fate in ILC3.
From: Hypoxia enhances ILC3 responses through HIF-1α-dependent mechanism
Analysis of HIF-1 interaction with genes using the chromatin immunoprecipitation (ChIP) technique. MNK3 cells were incubated for 3 h in hypoxia or normoxia without stimulation and then HIF-1α-associated chromatin sites were purified and amplified by qPCR (n = 3). A Measurement of HIF-1α binding to HIF-1α-responsive genes under hypoxia. B Measurement of HIF-1α binding to known HIF-1-binding or non-binding sites of Rorc. C Measurement of HIF-1α binding to the RORγt-flanked or not flanked sites of Il17. D Measurement of HIF-1α binding to Il22 and non-RORγt-flanking Dnajb6 and Egln3 genes. All ChIP-qPCR data were normalized by log2-fold change of total chromatin extraction (input) to purified immunoprecipitated chromatin. Results are representative of two independent experiments and presented as mean ± SEM. *p < 0.05; **p < 0.01.
