Fig. 3: CK2α controls Th1 differentiation through the IL-12/STAT4 pathway.
From: Protein kinase 2 (CK2) controls CD4+ T cell effector function in the pathogenesis of colitis
a Naive CD4+ T cells from CK2αfl/fl or CK2αfl/fldLck-Cre mice were polarized under Th1 conditions [anti-CD3 (1 μg/ml), anti-CD28 (1 μg/ml), IL-12 (10 ng/ml), and anti-IL-4 (10 μg/ml)] for 3 days. IFN-γ production by CD4+ T cells was detected by intracellular staining. Representative flow cytometry profiles of IFN-γ production by CD4+ T cells is shown (n = 5). b IFN-γ in the supernatant was measured by ELISA (n = 3). c Naive CD4+ T cells from CK2αfl/fl mice or CK2αfl/fldLck-Cre mice were activated with anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 24 h, stimulated with IL-12 (10 ng/ml) for the indicated time points, and then phosphorylated P-STAT4 Y693 in CD4+ T cells was detected by immunoblotting. d Relative ratio of phosphorylated P-STAT4 Y693 to total STAT4 at the indicated time points is shown (n = 3). e Naive CD4+ T cells from CK2αfl/fl or CK2αfl/fldLck-Cre mice were activated with anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 24 h, and expression levels of IL-12Rβ1 and IL-12Rβ2 detected by flow cytometry. Representative line graphs are shown. f Quantitation of MFI of IL-12Rβ1 and IL-12Rβ2 expression is shown. n = 3 in each group. Bars represent the mean ± SD. *P < 0.05, ***p < 0.001, and ****p < 0.0001.
