Fig. 4: CK2α is essential for CD4+ T cell proliferation in vitro and in vivo.
From: Protein kinase 2 (CK2) controls CD4+ T cell effector function in the pathogenesis of colitis
a CFSE-labeled naive CD4+ T cells were activated with the indicated concentrations of anti-CD3 (0.2, 0.5, and 1.0 μg/ml) and 1.0 μg/ml of anti-CD28 Abs for 72 h, and proliferation was assessed by CFSE dilution. Representative line graphs are shown. b Proliferation index of CD4+ T cells is shown (n = 3). c A total of 1 × 106 CFSE-labeled naive CD4+ T cells from CK2αfl/fl or CK2αfl/fldLck-Cre mice were transferred into Rag1−/− mice by i.v. injection. Mice were sacrificed 5 days later, and proliferation of CD4+ T cells from the spleen was assessed by CFSE dilution. Representative line graphs are shown. d Frequencies of cells undergoing proliferation is shown. Data represent pooled results from three independent experiments. CK2αfl/fl, n = 6; CK2αfl/fldLck-Cre, n = 8. Bars represent the mean ± SD. **P < 0.01, ***p < 0.001, and ****p < 0.0001.
