Fig. 4: Primary mouse airway epithelial cells increase the expression of XO during RSV infection and induce inflammatory innate cytokines. | Mucosal Immunology

Fig. 4: Primary mouse airway epithelial cells increase the expression of XO during RSV infection and induce inflammatory innate cytokines.

From: Uric acid pathway activation during respiratory virus infection promotes Th2 immune response via innate cytokine production and ILC2 accumulation

Fig. 4

Cultures of primary airway epithelial cells (AECs) from naïve Balb/c mice were infected with RSV for 24 h and underwent different treatments. a Xanthine oxidase expression in RSV infected cells compared with uninfected cells. b, c TSLP, IL-33, and IL-25 and IFNβ expression in AECs infected with RSV treated with or without XOI. d, e CCL2 expression and production in in AECs infected with RSV treated with or without XOI. f, g IL-33 and CCL2 expression in AECs that were treated with uric acid, RSV or RSV+ uric acid. h, i IL-33 and CCL2 expression in AECs that were treated with murine recombinant IL-1β (IL-1β), RSV or RSV + IL-1β. j IL-33 expression in AECs that were treated with murine recombinant IFNβ (IFNβ), RSV or RSV + IFNβ. k IFNβ expression in AECs that were treated with murine recombinant IL-33 (IL-33), RSV, or RSV + IL-33. Data represents the Mean ± SE from 4 replicates per group (experimental repeats 3–4). *p ≤ 0.05, **p ≤ .01, ***p ≤ 0.001.

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