Fig. 5: Erlotinib alleviates mice allergic airway inflammation. | Mucosal Immunology

Fig. 5: Erlotinib alleviates mice allergic airway inflammation.

From: EGFR activation-induced decreases in claudin1 promote MUC5AC expression and exacerbate asthma in mice

Fig. 5

a Flow charts of the HDM-induced asthma mouse model and erlotinib was administered during day 14–day 23. b The expression of p-EGFR was detected by immunohistochemistry (scale bar 50 μm; original magnification: ×400; n = 3). c Airway resistance was measured at baseline and in response to increasing doses of nebulized methacholine in anesthetized mice placed on a DSI Buxco PFT controller system. Data were derived from 3–5 animals per group. d, e The number of eosinophils in blood and total numbers of cells in BALF were counted. Data were derived from seven animals per group. f Hematoxylin and eosin -stained lung tissue sections (n = 3; scale bar: 50 μm; magnification: ×400). g The levels of IL-4 and IL-13 in BALF were detected by ELISA (n = 7). h, i The expressions of claudin1 and MUC5AC were detected by IHC and assessed by IPP software (scale bar: 50 μm; magnification: ×400; n = 3). Statistical comparisons were performed using one-way analysis of variance with Dunnett’s test and two-way analysis of variance with Bonferroni’s multiple comparison (#P < 0.05; ##P < 0.01; ###P < 0.001; ####P < 0.0001 vs control group, *P < 0.05; **P < 0.01; ***P < 0.001 vs the HDM group).

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