Fig. 5: Nmp4 deficient mice showed reduced innate immune response to influenza virus infection.

a Total viable cells in lung tissues after collagenase digestion. b Flow cytometric analysis of inflammatory monocytes/macrophages in lung tissues. c Flow cytometric analysis of neutrophils in lung tissues. d Flow cytometric analysis of eosinophils in lung tissues. e Flow cytometric analysis of monocytes and neutrophils in bone marrow. f Flow cytometric analysis of monocytes and neutrophils in blood. g Realtime PCR detection of Ccl2 expression in the lung tissue and ELISA measurement of CCL2 concentration in BALF. h Realtime PCR detection of Cxcl1 expression in the lung tissue and ELISA measurement of CXCL1 concentration in BALF. i Realtime PCR detection of Ccl7 expression in the lung tissue. Data represent mean ± SEM from one of four (a–d) or two (e–i) independent experiments. n = 10–12 mice for (a–d) and (g), and n = 4 mice for (e–f). *: p < 0.05; **: p < 0.01; ***: p < 0.001 by two-way ANOVA with Bonferroni post-test (a–g) or two-tailed t test (h, i).