Fig. 7: Ear11 mobilises peritoneal neutrophils.

a Naive LiveCD45⁺CD11b⁺ cells were sorted from blood, and stained with Ly6G and SiglecF to show the presence of neutrophils (Ly6G^highSiglecF⁻). b Sorted LiveCD45⁺CD11b⁺ cells were cultured in an in vitro transwell system with or without 20 ng/ml Ear11 and flow cytometry performed on cells which migrated through the membrane to show the percentage of Ly6G^highSiglecF⁻ migratory neutrophils. c Peritoneal lavage cells sorted from PBS- or Ear11-treated mice as LiveCD45⁺CD11b⁺. Top panel shows light blue cells CD11b⁺Ly6G^int and dark blue cells CD11b⁺Ly6G^high. Bottom panel shows neutrophils gated as Ly6G^highSiglecF⁻ within LiveCD45⁺CD11b⁺ gate. d Sorted cells were cultured as in b. e Total number LiveCD45⁺ migratory neutrophils collected following Ear11 treatment. Data are representative of two independent experiments.