Fig. 3: Increased proportion of Tregs in the small intestinal GALT of Zfp36^−/− mice.

Flow cytometry and RTqPCR results in WT or Zfp36^−/− mice. a Proportion (left) of Th17 cells (RORγt⁺ cells) among Foxp3⁻ CD4 T cells in SI LP (n = 12 mice/group, pooled from three independent experiments) and absolute number of Th17 in SI LP (right) (n = 6–7 mice/group, pooled from two independent experiments). b Proportion of IL-17 producers among total CD4 T cells (n = 4 mice/group). c Il17a gene expression in total ileum (n = 10–12 mice/group, pooled from three independent experiments). d Proportion of Tregs (Foxp3⁺ cells) among CD4 T cells in spleen, MLN, SI LP (n = 16 mice/group, pooled from five independent experiments), and colon lamina propria (C LP) (n = 7–9 mice/group, pooled from three independent experiments). e Absolute number of Tregs (Foxp3⁺ CD4⁺ T cells) in SI LP (n = 8–9 mice/group, pooled from three independent experiments). f Proportion of IL-10 producing Foxp3⁺ Treg in spleen, MLN, and SI LP (n = 4–6 mice/group, pooled from two independent experiments). g Il10 gene expression in total ileum (n = 23 mice/group, pooled from five independent experiments). h Proportion of Foxp3⁺ subsets (GATA3⁺, RORγt⁺ and GATA3⁻RORγt⁻ cells) among CD4 T cells (n = 7–8 mice/group, pooled from two independent experiments). RTqPCR results are expressed in arbitrary units, normalized against Actb mRNA levels and relative to the WT group. Results are given as median ± interquartile range and each dot represents a single mouse. Statistical significance (ns nonsignificant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was assessed by Mann–Whitney test (a–c, e, g) or two-way ANOVA test (d, f, h).