Fig. 1: Mouse models of acute local and systemic eosinophilic infiltration.

a Experimental timeline. Mice were sensitized with three intraperitoneal injections of OVA-alum followed 1 week later by three consecutive challenges through epicutaneous, lower airways, or oral routes with OVA. Sham control mice were sensitized with PBS-alum followed by challenges with PBS. Mice were sacrificed 4 days after the final allergen challenge and eosinophils quantified from the site of allergen challenge (b–e) or peripheral blood (f–h). b Eosinophils were quantified from H&E-stained sections of the dermis following allergen administration to tape-stripped skin. Following allergen administration to the lower airways lung tissue sections revealed cellular infiltrates (c) and induction of BAL fluid eosinophilia, as quantified by flow cytometry (d). e Oral gavage of allergen elicited increased numbers of eosinophils within intestinal jejunum, as quantified from αMBP immunohistochemistry. Allergen challenge to the skin (f), lung (g), or gut (h) also elicited an increase in peripheral eosinophils. Data shown are from one representative experiment (d) or combined data from two independent experiments (b, e, f–h). b, e Magnification ×400, scale bar 80 μm. c ×200, scale bar 200 μm. Error bars show standard deviation. **p < 0.001; ***p < 0.0001.