Fig. 2: Epicutaneous or endotracheal allergen challenge elicits an increase in eosinophils within remote, allergen nonexposed intestine.

Eosinophils were quantified from anti-MBP-stained jejunum tissue sections recovered 4 days after the final allergen challenge to tape-stripped skin (a) or lower airways (b). Frequencies of eosinophils (c), CD4⁺ T cells (d), CD8⁺ T cells (e), and dendritic cells (f) were further quantified by flow cytometry of single-cell suspensions of intraepithelial (IE, upper panels) or lamina propria (LP, lower panels) compartments recovered from whole small intestine using the following gating strategies: eosinophils, live, CD45⁺SSC^hiSiglecF^hi; CD4⁺ T cells, live, CD45⁺CD3⁺CD4⁺ cells; CD8⁺ T cells, live, CD45⁺CD3⁺CD8⁺ cells; and dendritic cells, live, CD45⁺SiglecF^-CD11c⁺ cells and expressed as percentage of CD45⁺ leukocytes. g Gated eosinophils from IE and LP preparations were further assessed for their expression of surface CD11c (left panel) and CD11b (right panel) that distinguish resident IE and LP eosinophils. Data are expressed as average change in mean fluorescence intensity (MFI) of relevant over isotype control antibodies. c–f N ≥ 4. Sham control mice were sensitized with PBS-alum and challenged with PBS. In a magnification ×200; scale bars, 100 μm. Error bars show standard deviation. *p < 0.01; **p < 0.001.