Fig. 3: Epicutaneous or intragastric allergen challenge elicits an increase in eosinophils within remote, allergen nonexposed lung.

a, b Eosinophils were quantified from anti-MBP-stained nonperfused (a) or PBS-perfused (b) lung tissue sections recovered 4 days after the final allergen challenge to tape-stripped skin (a) or gut (b). Combined data from two independent experiments are shown. PBS-perfused lungs from mice 24 h after final allergen challenge were digested and frequency of lung eosinophils determined by flow cytometry (c). Sham control mice were sensitized with PBS-alum and challenged with PBS. a, b Magnification ×200; scale bar, 100 μm. BV blood vessel, Aw airway. Error bars show standard deviation. *p < 0.05; **p ≤ 0.005.