Fig. 5: Impact of RA and ligand-binding ability on nuclear versus cytoplasmic RARα localization. | Mucosal Immunology

Fig. 5: Impact of RA and ligand-binding ability on nuclear versus cytoplasmic RARα localization.

From: A ligand-independent fast function of RARα promotes exit from metabolic quiescence upon T cell activation and controls T cell differentiation

Fig. 5: Impact of RA and ligand-binding ability on nuclear versus cytoplasmic RARα localization.

a Domains and the mutation site for G303E in the RARα protein. b Confocal images of FLAG-RARα expressed in CD4+ T cells. FLAG-RARα was expressed in CD4+ T cells by retroviral gene transfer. Naïve CD4+ T cells were activated overnight and transduced with recombinant retrovirus particles and further cultured in a Th17 cell cytokine condition for 16 h without or with RA (10 nM). c Cytoplasmic localization of FLAG-RARα as a percentage of total cell signal was measured using ImageJ. *Significant differences (P values < 0.05) from indicated groups by two-way ANOVA with Bonferroni (c).

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