Fig. 3: Brg1 facilitates the conversion of NKp46⁻ILC3s to NKp46⁺ILC3s by promoting T-bet expression.

a, b SI and LI LPLs were isolated from 14-day-old Rag1^−/−Smarca4^f/f or Rag1^−/−Smarca4^ΔILC3 mice. (n = 9; representative of three experiments). a Representative flow cytometry plots for NKp46⁺ILC3s (Lin⁻RORγt⁺NKp46⁺) and NKp46⁻ILC3s (Lin⁻RORγt⁺NKp46⁻) gated on Lin⁻ cells. b Frequencies of NKp46⁺ILC3s and NKp46⁻ILC3s in Lin^– cells. c, d Mixed bone marrow chimeric mice were constructed as in Fig. S2, except for that three of the biological repeats were constructed using Rag1^−/− instead of Rag2^−/−Il2rg^−/− mice as recipients. Mice were sacrificed for analyses 6 weeks later. (n = 6; representative of two experiments). c Representative flow cytometry plots showing percentages of NKp46⁺ILC3s among total Thy1⁺ILC3s in SI LPL from indicated donors. d Frequencies of NKp46⁺ILC3s in ILC3s from the small intestine of Rag1^−/−Smarca4^f/f or Rag1^−/−Smarca4^ΔILC3 donor mice. e Relative expression of Tbx21 in NKp46⁺ILC3s (CD45^lowThy1.2^highNKp46⁺) and NKp46⁻ILC3s (CD45^lowThy1.2^highNKp46⁻) sorted from the SI LPLs of Rag1^−/−Smarca4^f/f or Rag1^−/−Smarca4^ΔILC3 mice (n = 6, representative of two independent experiments). f–h SI LPLs were isolated from Rag1^−/−Smarca4^f/f or Rag1^−/−Smarca4^ΔILC3 mice. f Representative flow cytometry plots for T-bet⁺ILC3s gated on Lin⁻RORγt⁺NKp46⁻CCR6⁻ ILC3s. g Frequencies of T-bet⁺ILC3s in Lin⁻RORγt⁺NKp46⁻CCR6⁻ ILC3s. h Total numbers of NKp46⁻CCR6⁻T-bet⁺ ILC3s. i–k SI LPLs were isolated from Smarca4^f/f or Smarca4^ΔILC3 mice. (n = 6; representative of two experiments). i Representative flow cytometry plots for T-bet⁺ILC3s gated on Lin⁻RORγt⁺NKp46⁻CCR6⁻ ILC3s. j Frequencies of T-bet⁺ILC3s in Lin⁻RORγt⁺NKp46⁻CCR6⁻ ILC3s. k Total numbers of NKp46⁻CCR6⁻T-bet⁺ ILC3s. l, m Sorted NKp46⁻ILC3s were cultured on OP9-GFP or OP9-delta like canonical Notch ligand 4 (DLL4) for 7 days. (n = 7–9; representative of three experiments). l Representative flow cytometry plots for NKp46⁺ILC3s (NKp46⁺CD45^lowThy1.2^high) on ILC3s (CD45^lowThy1.2^high cells). m Frequencies of NKp46⁺ILC3s in ILC3s. a–m Data are represented as means ± SEM. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Lin⁻, CD3ε⁻B220⁻CD11b⁻CD11c⁻. DN ILC3s, Lin⁻RORγt⁺NKp46⁻CCR6⁻ ILC3s.