Fig. 6: Deletion of Brg1 endows ILC3s with pro-inflammatory property of producing GM-CSF.
From: Brg1 restrains the pro-inflammatory properties of ILC3s and modulates intestinal immunity
a–c LPLs isolated from Rag1−/−Smarca4f/f or Rag1−/−Smarca4ΔILC3 mice were stimulated with PBS, IL-23 (10 ng/ml), IL-1β (10 ng/ml) or TL1A (10 ng/ml) overnight or PMA plus ionomycin for 3 h prior to analyses. (n = 5–8; representative of four experiments). a Representative flow cytometry plots for GM-CSF (a) expression gated on ILC3s (CD45lowThy1.2highRORγt+) in SI LPLs. b, c Frequencies of GM-CSF+ cells in ILC3s from SI (b) and LI (c) LPLs. d–g 4–6-week-old Rag1−/−Smarca4f/f or Rag1−/−Smarca4ΔILC3 mice were treated with 250 μg of IgG or α-GM-CSF per mouse every 3 days for 14 days before analyses. (n = 12–14; representative of four experiments). d Representative H&E staining of colon sections (magnification ×10). Scale bar is 100 μm. e Histological scores of colon sections. f Representative flow cytometry plots for neutrophils (CD11b+Ly6G+) gated on live cells in LI LPLs. g Frequencies and total numbers of neutrophils in LI LPLs. a–g Data are represented as means ± SEM. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.001. Lin−, CD3ε−B220−CD11b−CD11c−.
