Fig. 3: Deficiency of Adam15 in mice increases CS-induced lung inflammation. | Mucosal Immunology

Fig. 3: Deficiency of Adam15 in mice increases CS-induced lung inflammation.

From: A disintegrin and metalloproteinase domain-15 deficiency leads to exaggerated cigarette smoke-induced chronic obstructive pulmonary disease (COPD)-like disease in mice

Fig. 3

Wild-type (WT) and Adam15−/− mice were exposed to air or CS for 1 month (mo), 3 months or 6 months. Absolute numbers of all leukocytes (a), macrophages (b), and lymphocytes (c) were counted in bronchoalveolar lavage (BAL) samples. Data are mean ± SEM; n = 12–14 mice exposed to air and 6–11 mice exposed to CS. Data were analyzed using a one-way ANOVA followed by pair-wise testing with two-tailed Student’s t tests. *P ≤ 0.005 versus the same genotype exposed to air or the group indicated. d, h WT and Adam15−/− mice were exposed to air or CS for 1 or 3 months, lungs were enzymatically digested, and leukocytes were immunostained for markers of lymphocyte subsets including CD4+ lymphocytes (d), CD8+ lymphocytes (e), CD3-positive NKT cells (f), Tregs (g), and B cells (h). Lymphocyte subsets were quantified using flow cytometry. Data are mean ± SD; n = 5 mice exposed to air and 8–9 mice exposed to CS. Data were analyzed using a one-way ANOVA followed by pair-wise testing with two-tailed Student’s t tests. *P < 0.05 air-exposed mice belonging to the same genotype or the group indicated.

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