Fig. 4: MHCII expressed by CCR10⁺ IgA-ASCs is not important for their formation of conjugates with T cells but is crucial for their establishment in the colon.

a Representative flow cytometric analysis of gated EGFP⁺ intracellular IgA⁺ cells of the PP, mLN, and colons for their MHCII^+/+CCR10^+/− (+/+) versus MHCII^−/−CCR10^+/− (−/−) donor B-cell origin and interacting CD4⁺ T cells in μMT recipient mice two weeks after transfer. Inclusion of one CCR10-KO/EGFP-KI allele (CCR10^+/−) in the donor B cells was to report the CCR10 expression with EGFP. b Relative percentages of CCR10⁺ IgA-ASCs of MHCII^+/+CCR10^+/− versus MHCII^−/−CCR10^+/− donor B-cell origin (left panel) and interacting CD4⁺ T cells (right panel) in the PP, mLN, and colons of μMT recipients 2 weeks after transfer. Relative percentages of contribution of MHCII^+/+CCR10^+/− versus MHCII^−/−CCR10^+/− donor B cells to IgA-ASCs were normalized on ratios of MHCII^+/+CCR10^+/− versus MHCII^−/−CCR10^+/− donor B cells in spleens of recipients. The dashed line indicates the 50% of relative contribution from each donor origin if MCHII^+/+CCR10^+/− and MHCII^−/−CCR10^+/− donor cells contribute equally. Seven recipient mice were analyzed.