Fig. 2: Reduced IL-6Rα expression in PTPN2-knockdown macrophages. | Mucosal Immunology

Fig. 2: Reduced IL-6Rα expression in PTPN2-knockdown macrophages.

From: Loss of protein tyrosine phosphatase non-receptor type 2 reduces IL-4-driven alternative macrophage activation

Fig. 2: Reduced IL-6Rα expression in PTPN2-knockdown macrophages.

THP-1 cells expressing non-targeting control (shCtr) or PTPN2-specific (shPTPN2) shRNA were differentiated into macrophages and treated for 24 h (A + B) or the indicated time (C–E) with 20 ng/ml IL-6 and analyzed for a IL6RA and b GP130 mRNA levels, as well as c–e IL-6Rα and GP130 protein levels. * = p < 0.05, *** = p < 0.001 relative to the same condition in shCtr cells, ANOVA with Dunnett’s multiple comparisons test; representative results from one out of three independent experiments with n = 5 (A + B) or n = 3 (C–E) independent replicates.

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