Fig. 5: IL-36R signalling promotes the generation of murine pro-inflammatory CD4+ T cells with a gut homing phenotype.
From: IL-36 cytokines imprint a colitogenic phenotype on CD4+ T helper cells
Flow cytometric analysis of transferred CD4+CD25−CD45Rbhi effector T cells from donor wt and Il36r−/− mice for surface expression of α4β7 (a and c) and CCR9 (b and d)(n = 4–5 mice per group). Statistical analysis performed by Mann–Whitney U Test, *p < 0.05. For in vitro analysis, wild type splenic CD4+ T cells were magnetically purified and activated with plate bound anti-CD3 and anti-CD28 and stimulated with the indicated IL-1 family cytokines; IL-36α (200 ng/ml), IL-18 (200 ng/ml), IL-1β (200 ng/ml), IL-33 (200 ng/ml); +/− ATRA (10 nM). Cells were incubated at 37 oC for 72 h and analysed for expression of α4β7 (e,f) by flow cytometry. Cumulative data from two indpendent experiments is shown (n = 8). Statistical analysis performed by Students T Test, *p < 0.05, **p < 0.01.
